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Run monocle2 on Seurat object

Usage

RunMonocle2(
  SeuratObj,
  max_components = 2,
  root_clusters = NULL,
  point_size = 0.5,
  BEAM_analysis = FALSE,
  BEAM_branch_point = 1,
  save_plot = TRUE,
  return_object = TRUE
)

Arguments

SeuratObj

A Seurat object

max_components

the dimensionality of the reduced space. 2 as default.

root_clusters

clusters selected as root

point_size

point size of scatter plot

BEAM_analysis

perform BEAM analysis or not.

BEAM_branch_point

The ID of the branch point to analyze.

save_plot

save plots to pdf or not.

return_object

return CellDataSet object or not.

Value

if parameter return_object = TRUE, CellDataSet object and differential genes across pseudotime will be returned.